Table 3 CRISPR Cas9 mutation frequencies in mammalian cell lines
| Cell line | G418-resistant isolates screened by western blot analysis | Number of homozygous truncation mutants | Homozygous mutation frequency (%) |
|---|---|---|---|
| U87MG Trial 1 | 77 | 4a | 5 |
| U87MG Trial 2 | 50 | 3a | 6 |
| BT549 | 56 | 5 | 9 |
| HEK293 | 77 | 1 | 1.3 |