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Table 5 Most stable reference genes and optimum number of reference genes calculated by geNorm and NormFinder

From: Predator-induced defences in Daphnia pulex: Selection and evaluation of internal reference genes for gene expression studies with real-time PCR

  geNorm NormFinder
   1stanalysis 2ndanalysis
  Tbp/CAPON Xbp1 Xbp1
  Xbp1 Tbp Tbp
  Stx16 CAPON CAPON
  GAPDH Stx16 Stx16
  aTub aTub  
  MMP GAPDH  
   MMP  
Optimum number 3 2 2
  1. Genes are ranked according to Figure 1A (geNorm) and to their stability values in Table 3 and 4, respectively (NormFinder). In bold letters: The recommended optimum combination of genes.
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BMC Molecular Biology

ISSN: 1471-2199

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